ANTIMETABOLITE ACTIVITY OF URIDINE AND CYTIDINE DERIVATIVES

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Antimetabolite Activity of Uridine and Cytidine

With the realization in 1940 (1) that structural analogues of a metabolite frequently prevent utilization of that metabolite, it became possible to develop antimetabolites which block specific biosynthetic pathways. By blocking various steps in a biosynthetic pathway, antimetabolites are useful for elucidating intermediary metabolism. It was the object of the present study to investigate the bi...

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Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases.

Uridine-cytidine kinases (UCK) have important roles for the phosphorylation of nucleoside analogs that are being investigated for possible use in chemotherapy of cancer. We have cloned the cDNA of two human UCKs. The approximately 30-kDa proteins, named UCK1 and UCK2, were expressed in Escherichia coli and shown to catalyze the phosphorylation of Urd and Cyd. The enzymes did not phosphorylate d...

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Phosphorylation of Uridine and Cytidine Nucleoside Analogs by Two Human Uridine-Cytidine Kinases

Uridine-cytidine kinases (UCK) have important roles for the phosphorylation of nucleoside analogs that are being investigated for possible use in chemotherapy of cancer. We have cloned the cDNA of two human UCKs. The '30-kDa proteins, named UCK1 and UCK2, were expressed in Escherichia coli and shown to catalyze the phosphorylation of Urd and Cyd. The enzymes did not phosphorylate deoxyribonucle...

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Metabolism of cytidine and uridine in bean leaves.

The metabolism of cytidine-2-(14)C and uridine-2-(14)C was studied in discs cut from leaflets of bean plants (Phaseolus vulgaris L.). Cytidine was degraded to carbon dioxide and incorporated into RNA at about the same rates as was uridine. Both nucleosides were converted into the same soluble nucleotides, principally uridine diphosphate glucose, suggesting that cytidine was rapidly deaminated t...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1952

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)55823-3